Chemical reactivity of RNA and its modifications with hydrazine.

• Tác giả: Katja Hollnagel, Stefanie Kaiser, Steffen Kaiser, Chengkang Li, Yuyang Qi, Leona Rusling, Helena Stafflinger, Jens Wöhnert, Nur Yeşiltaç-Tosun
• Ngôn ngữ: eng
• Ký hiệu phân loại: 579.25 *RNA viruses
• Thông tin xuất bản: England : Communications chemistry , 2025
• Bộ sưu tập: NCBI
• ID: 102739

Tóm tắt

RNA modifications are essential for the regulation of cellular processes and have a key role in diseases such as cancer and neurological disorders. A major challenge in the analysis of RNA modification is the differentiation between isomers, including methylated nucleosides as well as uridine and pseudouridine. A solution is their differential chemical reactivity which enables isomer discrimination by mass spectrometry (MS) or sequencing. In this study, we systematically determine the chemical reactivity of hydrazine with RNA and its native modifications in an aniline-free environment. We optimize the conditions to achieve nearly full conversion of all uridines while avoiding RNA cleavage. We apply the conditions to native tRNA