Recently, the number of carbapenem-resistant Citrobacter spp.(CRC) have been on the rise. In this study, we collected CRCs from humans, animals, and water environments to examine their epidemiological distribution and resistance characteristics. Total of 171 CRCs were isolated from diverse Chinese provinces between 2016 and 2023. All strains are classified into seven Citrobacter species, C. freundii is the most common (76.02%), with ST116 and ST22 being the predominant strains. Among all strains, 63.16% carried bla