pH-/Viscosity-Activatable NIR Fluorescent Probes via Acceptor Engineering of Hemicyanine Dyes for High-Contrast Bioimaging.

• Tác giả: Da Feng, Li Guo, Yuan Guo, Lulu Ning, Fang Yuan, Jianjian Zhang, Yihan Zhao
• Ngôn ngữ: eng
• Thông tin xuất bản: United States : Analytical chemistry , 2025
• Bộ sưu tập: NCBI
• ID: 106033

Tóm tắt

Real-time monitoring of the lysosomal microenvironment using small-molecule probes is critical for understanding the complex interactions between organelles and diseases associated with lysosomal dysfunction. Most traditional fluorescent-dye-based lysosome probes rely on the protonation of the nitrogen atom in the morpholine unit to visualize the lysosomal microenvironment by inhibiting the photoinduced electron transfer (PET) effect. However, these probes often face selectivity issues within the complex cellular microenvironment. For instance, classic hemicyanine dyes (HD) show nonspecific fluorescence responses in the liver due to inappropriate p