INTRODUCTION: The prevalence, clinical characteristics, and antibiotic susceptibility of the slow-growing microorganisms MATERIAL AND METHODS: Descriptive and retrospective cross-sectional study carried out in a Regional Hospital. Reports of clinical episodes with single isolation of the aforementioned microorganisms were selected between January 2016 and December 2023. RESULTS: A total of 186 single-isolation episodes were included, corresponding to 0.44% of the total. The most frequently isolated species was CONCLUSIONS: Slow-growing opportunistic emerging microorganisms represent a small percentage of the aetiological agents in urinary tract infections, although of important and growing clinical interest given their pathogenic role in the presence of certain circumstances and risk factors, as well as the challenges they pose for laboratory diagnosis. It is essential to perform antibiograms for an adequate targeted treatment.