The remediation of light non-aqueous phase liquids (LNAPLs) from groundwater remains a critical environmental challenge, particularly in heterogeneous aquifers. Existing remediation techniques often struggle with low-permeability zones, limiting their effectiveness. In this study, we developed and validated a microfluidic platform to simulate heterogeneous porous media and evaluate the performance of four chemical agents, including the green bio-solvent Cyrene. Cyrene demonstrated superior efficiency, achieving a 30-45 % increase in oil removal compared to conventional agents, particularly in low-permeability zones. The platform enabled real-time visualization of pore-scale displacement processes, while the gray value method quantified residual oil saturation (S