Separation of full and empty adeno-associated virus particles using a novel multi-modal anion exchange membrane.

• Tác giả: Xiaolei Hao, Ronny Horax, Xianghong Qian, S Ranil Wickramasinghe
• Ngôn ngữ: eng
• Ký hiệu phân loại: 025.30285572 Bibliographic analysis and control
• Thông tin xuất bản: Netherlands : Journal of chromatography. B, Analytical technologies in the biomedical and life sciences , 2025
• Bộ sưu tập: NCBI
• ID: 116345

Tóm tắt

A novel multi-modal anion exchange (MMAEX) membrane with both electrostatic and hydrophobic interaction moieties was developed for the separation of full and empty AAV2 capsids with only slight differences in surface charge and hydrophobicity. Both gradient and two-step elution have been able to separate the full and empty capsids effectively. During gradient elution with slight increase in conductivity coupled with weakening electrostatic and enhanced hydrophobic interactions between the ligand and the capsids, two distinctive elution peaks representing empty and full capsids were resolved. Full capsid recovery of 94 % at 89 % purity has been achieved with a loading density of ∼10