BACKGROUND: Bacterial infections remain a major challenge in the treatment of infected wounds due to their resistance. Novel agents are urgently needed for effective treatment. This study aimed to evaluate the effects of carbazole nanocubosomes on the healing of methicillin-resistant Staphylococcus aureus (MRSA)-infected full-thickness wounds. MATERIALS AND METHODS: Carbazole (Carbz) and cubosome/carbazole (Cubo/Carbz) structures were synthesized, and their physicochemical properties, toxicity, and in vitro antibacterial activities were assessed. The wound healing activity was evaluated by analyzing pathological parameters, including edema, immune cell infiltration, new vessel formation, fibroblast infiltration, and epithelial thickness. Additionally, the expression levels of TNF-α, Bcl-2, P-53, mTOR, cyclin D1, TGF-β, VEGF, and Col1A were measured to assess the effects of cubosomes on the wound healing process. The results confirmed the successful synthesis of cubosomes, along with the safety, antibacterial activity, and sustained release of Carbz. RESULTS: The administration of Carbz, particularly the Cubo/Carbz formulations, significantly accelerated the wound healing process by reducing bacterial counts, edema, immune cell infiltration, and the expression of Bcl-2 and TNF-α. Simultaneously, fibroblast infiltration, epithelial thickness, and the expression of P-53, mTOR, Col1A, cyclin D1, TGF-β, and VEGF were increased. CONCLUSION: In conclusion, Cubo/Carbz accelerates the wound healing process by promoting apoptosis, reducing inflammation, and enhancing the expression of proliferative genes.