Ring finger protein 146 (RNF146) participates in regulating ferroptosis and ferroptosis is involved in myocardial ischemia/reperfusion injury (MI/RI). However, the effects and mechanisms of RNF146 in MI/RI are still unclear. TTC, H&E, IHC, DHE stainings, and echocardiography technology were used to determine the myocardial infarction area, pathological injury, level of RNF146, ROS, and cardiac function parameters, respectively. CCK-8 was employed to determine the cell viability. The corresponding kits, RT-qPCR, and western blot were adopted to determine the levels of CK-MB, LDH, Fe