Normal and pathological states of cells can be distinguished by their mechanical properties, which are thought to be determined by the organization of the actin network. In the body, cells exist in both adherent and non-adherent (suspended) states, and studies of the rheological properties of spread and suspended cells are needed to gain more insight into their response to strain. Herein, we show that WM35 melanoma cells in adherent and non-adherent states respond differently to oscillatory strain. We used an atomic force microscopy (AFM)-based microrheological approach to study the elasticity and fluidity of the cells, quantified by the storage (G') and loss (G") moduli and the transition frequency f