Triadimenol, a triazole fungicide, induces various adverse effects including neurotoxicity, hepatotoxicity, and developmental/reproductive toxicity in non-target organisms. Occupational exposure generally occurs in male agricultural workers. Investigating the effects of triadimenol on three different testicular cell lines would be valuable in elucidating the mechanisms underlying male reproductive issues or infertility. This preliminary study examines the potential toxic effects of triadimenol exposure in Leydig (TM3), Sertoli (TM4), and mouse-derived Spermatogonia (GC-1) cell lines, which are representative of the male reproductive system in vitro. The median inhibitory concentration (IC