Rosa hugonis is widely distributed in the Hengduan Mountains, Qinling Mountains, and northern China. It is an important candidate species for ecological restoration, given its good adaptability. Here, we present the first high-quality chromosome-level assembly of R. hugonis based on HiFi reads and Hi-C data. The sequencing data were then assembled onto seven pseudochromosomes of R. hugonis. The genome sizes of R. hugonis is 337.92 Mb, with contig N50 length of 26.84 Mb. We annotated 36,218 protein-coding genes in R. hugonis. In summary, the high-quality genome sequences of R. hugonis provide a genetic roadmap for the study of its genetics and species relationships. This will facilitate future genomic comparative studies across more species within Rosa.