Although agrochemicals protect crops and reduce losses, these chemicals can migrate to non-target environments via run-off and leaching following irrigation or heavy rainfall, where non-target organisms can be exposed to a mixture of water-soluble compounds. This study investigated whether the water-soluble fractions of selected agricultural soils from South Africa contain quantifiable concentrations of four commonly used pesticides, 2,4-dichlorophenoxyacetic acid (2,4-D), atrazine, dicamba and imidacloprid, and whether the aqueous extracts induce effects in vitro. Effects investigated included cytotoxicity using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] cell viability assay
xenobiotic metabolism using the H4IIE-luc rat hepatoma cell line
and (anti-)androgenic and (anti-)oestrogenic effects were screened for with the human breast carcinoma cell lines MDA-kb2 and T47D-KBluc, respectively. Oxidative stress responses were measured in H4IIE-luc and human duodenum adenocarcinoma (HuTu-80) cells. All extracts of soil induced oxidative stress, while several samples caused moderate to severe cytotoxicity and/or anti-androgenic effects. The herbicide atrazine had the greatest frequency of detection (89%), followed by dicamba (84%), 2,4-D (74%) and imidacloprid (32%). Concentrations of atrazine [2.0 × 10