Effective electrocatalysts and electrodes are the core components of energy conversion and storage systems for sustainable carbon and nitrogen cycles to achieve a carbon-neutral economy. Two-dimensional conjugated metal-organic frameworks (2D c-MOFs) have emerged as multifunctional materials for electrochemical applications benefiting from their similarity to graphene with remarkable conductivity, abundant active sites, devisable components, and well-defined crystalline structures. In this review, the structural design strategies to establish active components with a maximum degree through redox-active ligand assembly in 2D c-MOFs are briefly summarized. Next, recent representative examples of 2D c-MOFs applied in electrocatalysis (hydrogen/oxygen evolution and oxygen/carbon dioxide/nitrogen reduction) and energy storage systems (supercapacitors and batteries) are introduced. The synergistic effect of multiple components in 2D c-MOFs is particularly emphasized for enhanced performance in electrochemical energy conversion and storage systems. Finally, an outlook and challenges are proposed for realizing more active components, elucidating the reaction mechanism involving the derived structures, and achieving low-cost economy in practical applications.