The purpose of this research is to examine the relationship between the social well-being of healthcare professionals and their perception of gender roles. The correlational screening model was used in the research. The study group consisted of 247 healthcare professionals working in a university hospital. As a data collection tool in the research, the Social Well-Being Scale-Healthcare Professionals Version, Gender Roles Attitude Scale, and Demographic Data Form were used. Unrelated Samples t-test, One-Way Analysis of Variance, Pearson Product Moment Correlation Coefficient, and Multiple Linear Regression Analysis techniques were used to analyze the data. It was determined that the social well-being average of healthcare workers was 121.43 ± 24.46 and the gender role average was 99.48 ± 9.70. It was observed that the average social well-being of healthcare professionals differed at a statistically significant level according to family structure, length of service in the current institution, alcohol use, gender roles, gender, and profession (