Physiological resilience or durability is now recognised as a determinant of endurance performance such as road cycling. Reliable, ecologically valid and standardised performance tests in laboratory-based cycling protocols have to be established to investigate mechanisms underpinning, and interventions improving durability. This review aims to provide an overview of available race simulation protocols in the literature and examines its rigour around themes that influence durability including (i) exercise intensity anchoring and (ii) carbohydrate intake whilst also (iii) inspecting reliability and justification of the developed protocols. Using a systematic search approach, 48 articles were identified that met our criteria as a cycling race simulation. Most protocols presented limitations to be recommended as exercise test to investigate durability, such as not appropriately addressing the influence of exercise intensity domains by anchoring exercise intensity as % peak power or %