Calibrations in LA-ICP-MS are typically very time-consuming and complex, as they need to be matched to the samples being measured and sectioned on a microtome. Alternatively, gelatin can be in droplet form or as a section, which is a more recent development. In this study, we report on investigations where hot multi-element gelatin solutions are placed in a linear fashion on microscopic slides to conduct comparative statistical observations between doped tissue homogenates from the liver and lung. The tissue homogenates served as both samples (complete ablation) and calibration standards (partial ablation) for verification purposes. We explored the effects of different laser fluences (0.50-1.50 J/cm