Immunological Pre-Metastatic Niche in Dogs With Naturally Occurring Osteosarcoma.

• Tác giả: Kristin Paaske Anfinsen, David Argyle, Mikael Kerboeuf, Erling Olaf Koppang, Frode Lingaas, Lars Moe, Bente Kristin Sævik, Jon Teige
• Ngôn ngữ: eng
• Ký hiệu phân loại: 627.12 Rivers and streams
• Thông tin xuất bản: England : Veterinary and comparative oncology , 2025
• Bộ sưu tập: NCBI
• ID: 159929

Tóm tắt

Pre-metastatic niche (PMN) formation is essential for metastatic development and drives organotropism. Tumour-derived extracellular vesicles and soluble factors remodel the microenvironment of distant metastatic organs before subsequent metastasis. Dogs with osteosarcoma (OS) have proven to be excellent disease models for their human companions. Here, we show evidence of PMN formation in dogs with OS before metastasis. We necropsied and sampled lung tissues from dogs with naturally occurring treatment-naïve OS (n = 15) and control dogs without cancer (n = 10). We further divided dogs with OS into those having lung metastases (n = 5) and those without (n = 10). We stained formalin-fixed paraffin-embedded tissues using multiplex immunofluorescence to quantify the number of bone marrow-derived cells, monocytes and macrophages in the lung samples from each dog. The numbers of CD204