The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite that significantly threatens the poultry industry, not only through blood-feeding but also as a vector for deadly pathogens. With the growing challenge of acaricidal resistance, the demand for alternative control measures is urgent. However, effective PRM research, particularly in acaricidal efficacy and new drug discovery, hinges on the availability of reliable laboratory colonies. In this study, we successfully established a stable PRM laboratory colony, originally isolated from the field in 2021 and maintained under controlled conditions at the Research Institute for Animal Science in Biochemistry and Toxicology (RIAS). We investigated the growth kinetics and population dynamics of the laboratory colony within a Styrofoam-based maintenance box (SBMB) containing chicks. PRM propagation was tracked over 28 days, with mites collected every seven days. The average bulk weight of the mites in the trap increased from 4.3 ± 1.2 mg on day 7 to 201.4 ± 56.5 mg on day 28, despite seasonal variations, indicating optimal conditions for population growth. The collected mites spanned various blood-feeding developmental stages such as protonymph, deutonymph, and adult stages, enabling comprehensive assessments of molting and egg-laying efficiency. Our findings confirm that the laboratory colony of PRM can be stably maintained, providing a reliable source of PRMs for further experimental research aimed at advancing control strategies against this pervasive pest.