In recent years, the increasing prevalence of viral infections such as dengue (DENV) and chikungunya (CHIKV) has emphasized the vital need for new diagnostic techniques that are not only quick and inexpensive but also suitable for point-of-care and home usage. Existing diagnostic procedures, while useful, sometimes have limits in terms of speed, mobility, and price, particularly in resource-constrained environments and during epidemics. To address these issues, this study proposes a novel technique that combines 3D printing technology with electrochemical biosensors to provide a highly sensitive, user-friendly, and customizable diagnostic platform. This study focuses on a unique 3D-printed electrode cassette made with fused deposition modeling technology, which ensures strong structural alignment and improved performance under a variety of environmental conditions. When combined with paper-based electrodes loaded with silver nanoparticles, the platform dramatically enhances the detection sensitivity and reliability. The biosensor uses cyclic voltammetry and electrochemical impedance spectroscopy to detect DENV and CHIKV antigens within a linear range of 1 × 10