Wool is the most widely used proteinic natural fiber in the clothing industry by virtue of its versatile properties. Unfortunately, wool, as a natural fiber, is more susceptible to attack by microorganisms and moths, which may cause harm to the fiber and human health. That is why the antimicrobial and mothproof finishing of natural textiles is of prime importance to the textile and clothing industry. Herein, wool fabric was treated with the synthesized 6-aminocoumarin adopting the pad-dry-cure technique with or without a cross-linker. The treated wool fabric was evaluated for its antimicrobial activity against