Hemodialysis and bioartificial kidney (BAK), which mimic both physical and biological functions, can significantly impact chronic kidney disease (CKD) patients. Here we report on Hollow fiber membranes (HFMs) with enhanced separation of uremic toxins along with enhanced hemocompatibility and biocompatibility that also promote the growth of kidney cells. The improvement arises from the addition of titanium dioxide (0.1, 0.5, 2%) into poly(ether sulfone) (PES) HFMs during fiber spinning, resulting in enhanced growth and proliferation of HEK293 cells, which were able to form spheroids as evidenced by the confocal images. MTT cell viability assay, cell proliferation assay by cell count, live dead assay by flow cytometry, and reactive oxygen species (ROS) study showed metabolically active viable cells, a higher number of cells, and a low ROS level in TiO