Despite their widespread utilization in biomedical applications, these synthetic materials can be susceptible to microbial contamination, potentially compromising their functionality and increasing the risk of infection in patients. In this study, molybdenum (Mo), an essential metal in biological systems, was investigated as a Mo-based cold-sprayed coating on poly(dimethylsiloxane) (PDMS) for its potential use as biocompatible and antimicrobial surfaces for biomedical applications. Various cold-spray parameters were employed in the fabrication of Mo-embedded PDMS surfaces to alter the surface structure of the substrate, Mo loading density, and embedding layer thickness. Specifically, relatively low nozzle scanning speeds were used to develop high-density Mo-embedded PDMS surfaces. A comprehensive analysis was conducted to investigate how cold-spray processing parameters affect the surface topography, wettability, and chemical properties. The ability of the Mo-embedded PDMS to inhibit the colonization of