BACKGROUND: Cell culture studies play an important role in addressing fundamental scientific questions. However, inadequate reporting of these studies results in a lack of transparency and reproducibility. Recognizing the need for improvement, several ongoing efforts, such as CRIS guidelines and the ICLAC checklist, are focused on enhancing best practices for in vitro studies. Nonetheless, a comprehensive guideline specifically addressing the reporting of cell culture methods remains lacking. In this manner, a consensus-based approach is being undertaken to develop the Preferred and Transparent Reporting Items for Cell Culture Studies (PETRICCS) guideline. This project aims to present the protocol and the details for its development. METHODS: The process comprises five phases: (i) Initial Steps: a Steering Committee identifies the need for a guideline and drafts the PETRICCS protocol
(ii) Pre-meeting: an International Group of Cell Culture Experts (IGCE) reviews the draft guideline through a Delphi consensus exercise
(iii) Consensus Meeting: the steering committee presents the guideline's development, addresses concerns, and reaches consensus on the final items
(iv) Post-meeting: explanatory documents are prepared to assist authors in reporting their findings
and (v) Post-publication: PETRICCS, along with supporting documents, is published and made freely accessible. RESULTS AND CONCLUSIONS: PETRICCS will assist researchers in reporting and reviewing cell culture findings, enhancing transparency and reproducibility while filling a gap in this crucial scientific field. The guideline will incorporate the experiences of experts, creating a more equitable environment for authors, peer-reviewers, and editors during the publication process.