The T cell receptor (TCR) plays a crucial role in antigen recognition and signal transduction during T cell immunity. While the TCR locus has been well characterized in mammals, its knowledge in teleosts remains limited. In this study, we identified the TCRβ locus in large yellow croaker (Larimichthys crocea), an important mariculture species in China, and found 31 V, 2 D, 13 J, and 2 C gene segments. The 2 C gene segments are highly similar in amino acid sequences, and share conserved residues with TCRβ from other species. A consensus recombination signal sequence (RSS) is found to flank the V, D, and J gene segments, with conserved spacer lengths as observed in mammals. The V gene segments are consisted of a leader exon, an intron, and a V