The NAC transcription factor family is essential for plant growth, development, and stress responses. This study, based on RNA-Seq data from 84K poplar and weighted gene co-expression network analysis (WGCNA), identified PagNAC17 as a key factor in the salt stress response of poplar. A total of 202 PtrNAC TFs were identified and categorized into two major subfamilies, with their conserved motifs, gene structures, and cis-acting elements analyzed. Genes co-expressed with PagNAC17 are involved in energy metabolism, such as photosynthesis (e.g., light absorption and CO