For the development of stent-based intraluminal prostheses to treat chronic venous insufficiency, comprehensive knowledge of morphometric and mechanical parameters of the femoral veins (FV) is crucial for an optimized implant design. These data is essential as input for material models for numerical simulations of venous valves prostheses. However, data on mechanical properties of native/unfixed FV tissue are scarce. Unfixed native tissue, though, has limited availableness and durability due to progressing decay. To address these challenges, this study aimed to determine stress-strain behavior and compliance from native specimens of the human FV and its valves from body donations and compared the data to chemically fixed material. A large data set has been generated in tensile tests with a total of 313 venous wall and 78 valve leaflet specimens in different fixation groups from a total of 41 body donations. Tensile testing was conducted at cut rectangular specimens in longitudinal as well as circumferential direction of the vein walls and in one specific direction for the valve leaflets. Young's moduli E