BACKGROUND: The levels of free amino acids (FAAs) and the timing of bud flush (TBF) are among the the most economic traits of tea plants (Camellia sinensis). Investigating the genetic variation characteristics of FAAs and their potential associations with TBF is critical for the breeding of new tea cultivars with high economic value. METHODS: In this study, we utilized the 'Emei Wenchun' (♀) × 'Chuanmu 217' (♂) filial 1 (F RESULTS: A total of 16 FAAs were measured, and the average total FAA contents were 28.1 and 25.4 mg/g (dry weight) in the two spring seasons and 14.29 mg/g in the summer season. Within the population, the coefficients of variation (CV) for the FAAs ranged from 23 to 41% within each season, and the correlation coefficients (r) varied from 0.15 to 0.35 across seasons. ANOVA analyses revealed that 13 out of the 16 FAAs exhibited significant genetic variation, with the estimated broad-sense heritability (h CONCLUSION: Within the F