The Effects of Simulated Cobalt-Chromium-Molybdenum Wear Particles on a Macrophage-Lymphocyte Co-Culture for Evaluating Cellular Corrosion.

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Tác giả: Bailey Bond, Madison N Brown, Danielle M Bryant, William M Mihalko, Harrison Smith, Richard A Smith

Ngôn ngữ: eng

Ký hiệu phân loại: 673.734 Chromium, molybdenum, tungsten

Thông tin xuất bản: United States : The Journal of arthroplasty , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 165898

 BACKGROUND: This study examined the impact of simulated wear particles on inflammatory cell-induced corrosion (ICIC). METHODS: A 30-day macrophage-lymphocyte co-culture experiment was conducted using American Society of Testing and Materials F1537 cobalt-chromium-molybdenum (CoCrMo) disks, with activators and CoCrMo particles added at none, low (1:10), medium (1:100), and high (1:500) cell-particle ratios. Supernatants collected on days 10 and 30 were analyzed for tissue necrosis factor alpha (TNFα) and interleukin 6 (IL-6) levels via enzyme-linked immunosorbent assay. Disks were examined for ICIC damage using scanning electron microscopy (SEM), and the oxygen percentage on their surfaces was analyzed with energy-dispersive X-ray spectrometry (EDS) and X-ray photoelectron spectrometry (XPS). RESULTS: Most disks showed damage consistent with ICIC. Day 10 TNFα was higher in medium and high particle groups compared to groups without particles, while IL-6 was unexpectedly lower in those groups. On day 30, the activated medium particle group showed higher IL-6 than the non-activated group. The EDS showed no significant differences in %O (P = 0.77), but XPS results indicated significant differences (P <
  0.0001) at high particle concentrations. CONCLUSION: Overall, the data suggested that increased TNFα reflected a heightened inflammatory response, particles might temporarily inhibit IL-6 release, and there is likely a synergistic effect between activators and particles on cellular responses.
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