Recombinant enzymes have become increasingly popular and are frequently used as environmentally safe biocatalysts due to their wide range of applications and high specificity. Purifying these enzymes from the host cells, media, and other contaminants is essential for their characterization and applications. The widely utilized method for protein purification by nickel-nitrilotriacetic acid (Ni-NTA) resin-based affinity chromatography is a time-consuming, labor-intensive, and resource-demanding technique. In this study, we synthesized NTA-Ni@Fe