BACKGROUND: Total thyroxine (TT4) measurement is used to assess thyroid status in cats. OBJECTIVES: The aim of the prospective study was to evaluate the analytical performance of the point-of-care analyzer (POCA) Immuno AU10V using the v-T4 test kit for feline TT4 measurement. Additionally, method comparison with a benchtop analyzer (IMMULITE 2000) was done. METHODS: Validation included linearity, inter- and intra-assay precision, precision near the lower limit of quantification (LloQ), and interference testing for hemoglobin, lipid, and bilirubin. Correlation and bias were assessed. RESULTS: Linearity was given within the dynamic range. Coefficients of variation (CV) were ≤4% near the LloQ as well as for intra-and inter-assay precision. No interference was observed for lipid and bilirubin, while hemoglobin caused a negative bias of 28%. Method comparison included 74 samples within three TT4 concentration ranges (0.5-3.7, >
3.7-5.13, >
5.13-8 μg/dL). Correlation between POCA and reference method was excellent (r CONCLUSIONS: The analytical performance of the POCA was excellent, as was its correlation with the reference method. Except for the interferent effect of hemoglobin, the TE