AIM: This study compares retinal thickness measurements in healthy eyes using one SD-OCT and two SS-OCT devices to assess differences and consistency for clinical application. METHODS: Forty-eight eyes with a mean age of 28.15 ± 8.85 years were enrolled. Retinal thickness was measured using Heidelberg Spectralis SD-OCT, Svision VG200 SS-OCT, and TowardPi En Face SS-OCT. Normally distributed data were presented as mean ± SD
non-normal data as median (P25-P75). Intraclass correlation coefficients (ICC) and Bland-Altman analysis were used to assess agreement, with a 7 μm error threshold. RESULTS: Significant differences were found between the three devices ( CONCLUSION: The measurements of macular retinal thickness using SD-OCT and SS-OCT devices showed poor consistency and cannot be used interchangeably. However, measurements obtained from different SS-OCT devices demonstrated good consistency. To enhance the accuracy of results, it is recommended to maintain consistency in the devices used for follow-up examinations in the same patient.