INTRODUCTION: Vancomycin-resistant METHODS: Transcriptome analysis was performed on the V27 isolate, comparing cells in mature biofilms treated with and without GLA. The findings were further validated using qRT-PCR on six VRE-fm isolates and two RESULTS: Transcriptome analysis revealed a significant downregulation in the expression levels of genes associated with biofilm formation, including CONCLUSION: GLA effectively targets multiple aspects of biofilm formation in VRE-fm, including the downregulation of key biofilm-related genes, the inhibition of quorum sensing systems, and the modulation of metabolic pathways. GLA emerges as a promising candidate for eradicating