Isolation of Intact Mitochondria From

• Tác giả: Christopher M Groen, Anthony J Windebank
• Ngôn ngữ: eng
• Thông tin xuất bản: United States : Bio-protocol , 2025
• Bộ sưu tập: NCBI
• ID: 172264

Tóm tắt

Analysis of mitochondrial function has broad applicability in many research specialties. Neurodegenerative disorders such as chemotherapy-induced peripheral neuropathy (CIPN) often exhibit damaged mitochondria or reduced mitochondrial respiratory capacity. Isolation of intact mitochondria for protein analysis or respiration measurements has been previously reported in numerous model organisms. Here, we describe an adaptation of previous protocols to isolate intact functional mitochondria from