INTRODUCTION: Ratoon rice, as a high-efficiency rice cultivation mode, is widely applied around the world. Mechanical righting of rolled rice stubble can significantly improve yield in regeneration season, but lack of automation has become an important factor restricting its further promotion. METHODS: In order to realize automatic navigation of the righting machine, a method of fusing an instance segmentation model and a monocular depth prediction model was used to realize monocular localization of the rolled rice stubble rows in this study. RESULTS: To achieve monocular depth prediction, a depth estimation model was trained on training set we made, and absolute relative error of trained model on validation set was only 7.2%. To address the problem of degradation of model's performance when migrated to other monocular cameras, based on the law of the input image's influence on model's output results, two optimization methods of adjusting inputs and outputs were used that decreased the absolute relative error from 91.9% to 8.8%. After that, we carried out model fusion experiments, which showed that CD (chamfer distance) between predicted 3D coordinates of navigation points obtained by fusing the results of the two models and labels was only 0.0990. The CD between predicted point cloud of rolled rice stubble rows and label was only 0.0174.