OBJECTIVE: This study aims to incorporate contact-killing quaternary ammonium into two root canal sealers, AH Plus (DentSply Sirona, New York City, NY, USA) and BC (FKG, Le Crêt-du-Locle Switzerland) sealers to improve their antibacterial properties. METHODS: Dimethylaminohexadecyl Methacrylates (DMAHDM) were synthesized and incorporated into AH Plus and BC sealers at 5 weight percent (wt.%). The physical properties were assessed via film thickness, flow, contact angle, and solubility. The antibacterial properties were assessed by determining the number of colony-forming units (CFUs) of RESULTS: Incorporating DMAHDM at 5 wt.% increased the film thickness and reduced the flow of the AH Plus and BC sealers ( CONCLUSIONS: The results of this study suggest that DMAHDM, as a contact-killing agent, could be used as an approach to prevent endodontic reinfections. CLINICAL RELEVANCE: Integrating DMAHDM into commercial sealers may enhance their antibacterial properties. These findings indicate a need for further investigation using more clinically relevant models to validate this approach.