BACKGROUND/OBJECTIVES: Green MATERIALS/METHODS: The effects of GYE on adipocyte differentiation were assessed by measuring Oil red O staining, mRNA and protein expression. The beneficial effects of GYE on HFD-induced obese mice were evaluated using the body weight, body composition, visceral fat size, and biochemical analysis. RESULTS: GYE inhibited adipocyte differentiation and lipid accumulation compared to the control cells, as evidenced by Oil red O staining and the triglyceride level, respectively. GYE down-regulated the adipogenic genes CCAAT/enhancer binding protein α ( CONCLUSION: GYE suppressed adipocyte differentiation by inhibiting the PI3K-Akt pathway