Studies show that microorganisms resistant to numerous antibiotics spread infections, lengthen hospital stays, and increase fatalities. Amplification factors increase the demand for innovative drug-resistant disease-fighting chemicals. This research synthesised the chiral L-phenyl alanine condensed with a 2, 4-dihydroxy benzaldehyde Schiff base for biological efficacy investigations such as antimicrobial, antidiabetic, DPPH free radical, MTT assay against HeLa cells and hemolysis studies after the characterization. Derived Schiff base showed good inhibition against