BACKGROUND: Growing reports are dedicated to providing novel agents for wound healing with fewer adverse effects and higher efficacy. The efficacy of nanofibers composed of polyvinyl alcohol (PVA)/polyethylene oxide (PEO)/chitosan (CS) in promoting wound healing can be attributed to their ability to stimulate collagen production. Among the herbal agents with fewer adverse effects, PURPOSE: In the present study, our objective was to assess the wound-healing activity of PVA/PEO/CS nanofibers containing METHODS: Synthesized nanofibers from PVA, PEO, and CS were done by the electrospinning method and confirmed by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FT-IR). The release tests of nanofibers were assessed through the UV-visible method at different time intervals, which were conducted for about 60 h. To evaluate the wound healing effects, rats were divided into four distinct groups, including negative control (untreated), phenytoin cream (as positive control), polymer (PVA/PEO/CS), and drug (nanofiber-containing 50% of RESULTS: PVA/PEO/CS nanofibers containing 1.2 g of PVA, 0.3 g of PEO, and 0.8 g of CS, along with 50% of CONCLUSION: PVA/PEO/CS/