PURPOSE: Urinary incontinence (UI) is common in nulliparous female elite athletes, but underlying pathophysiology is inadequately understood. We examined urinary symptoms and associated pelvic floor anatomy and function in this population, hypothesizing that athletes with UI would exhibit pelvic floor findings seen in older incontinent women (eg, pelvic floor descent and urethral hypermobility) and findings attributable to elite sporting such as muscle hypertrophy. MATERIALS AND METHODS: We performed a cross-sectional study of National Collegiate Athletic Association Division 1 nulliparous female athletes comparing symptomatic (ie, currently or previously experienced urinary leakage during exercise) and asymptomatic athletes. We assessed demographics, sport characteristics, relevant medical history (including Brief Eating Disorder in Athletes Questionnaire) and validated genitourinary symptom questionnaires (Lower Urinary Tract Dysfunction Network Symptom Index-29 and Female Genitourinary Pain Index). Pelvic examination and dynamic MRI were used to assess the pelvic floor at rest and at maximal strain. Pelvic floor anatomic structures were evaluated and segmented by MRI. RESULTS: Of 29 athletes (11 asymptomatic, 18 symptomatic), demographics, sport characteristics, and pelvic examination findings were not statistically different between groups. Symptomatic athletes had significantly worse urinary symptoms (median LURN SI-29 8.5 vs 8.0, CONCLUSIONS: In nulliparous female elite athletes with UI, dynamic pelvic MRI identified differences in pelvic floor anatomy, which may be part of a multifactorial etiology of UI.