Plant extracts and bacterial biofilm are acknowledged to offer impressive corrosion-inhibitory activities. However, anticorrosive properties of their combination are still less reported. Thus, in the present study, we aimed to evaluate the corrosion inhibition efficiency of Saccharum officinarum bagasse (SOB) plant extract, Pseudomonas chlororaphis (P. chlororaphis), and Bacillus coagulans (B. coagulans) individually and in combination (SOB+P. chlororaphis) and (SOB+B. coagulans) using copper sheets in different acidic solutions (pH 5.5) and at three different temperatures (300 K, 305 K, and 310 K). The weight loss and electrochemical measurements were carried out to evaluate the corrosion rate and inhibition efficiencies. Alteration in the surface morphology of copper sheets was analyzed employing Fourier transform infrared (FTIR) spectroscopy, UV visible spectroscopy, scanning electron microscope (SEM), X-ray diffraction (XRD), energy dispersive X-ray (EDX), and X-ray photoelectron spectroscopy (XPS). Amongst all, SOB extract alone exhibited the highest anticorrosive efficiency, whereas the combination of SOB with P. chlororaphis revealed excellent inhibition efficiency. Weight loss studies indicated that at 300 K, the combination of SOB extract with P. chlororaphis significantly reduces the corrosion rate up to 11.90% with the highest inhibition efficiency of 88.09%. However, with an increase in temperature, i.e. 310 K, the corrosion rate diminishes to 13.04% with inhibition efficiency of 86.95% for 500 µL concentration in HNO