Low-coverage whole genome sequencing of low-grade dysplasia strongly predicts advanced neoplasia risk in ulcerative colitis.

• Tác giả: Ibrahim Al Bakir, Ann-Marie Baker, Hayley L Belnoue-Davis, Theo Clarke, George D Cresswell, Kit Curtius, Philip D Dunne, James E East, Jennifer Fisher, Trevor A Graham, Heather E Grant, Qingli Guo, Ailsa L Hart, Marnix Jansen, Christopher Kimberley, Simon J Leedham, Maurice B Loughrey, Morgan Moorghen, Maximilian Mossner, Nadia Nasreddin, Salpie Nowinski, Manuel Rodriguez-Justo, Kane Smith, Ally Speight, Nicholas A Wright
• Ngôn ngữ: eng
• Thông tin xuất bản: England : Gut , 2025
• Bộ sưu tập: NCBI
• ID: 177532

Tóm tắt

 BACKGROUND: The risk of developing advanced neoplasia (AN
  colorectal cancer and/or high-grade dysplasia) in ulcerative colitis (UC) patients with a low-grade dysplasia (LGD) lesion is variable and difficult to predict. This is a major challenge for effective clinical management. OBJECTIVE: We aimed to provide accurate AN risk stratification in UC patients with LGD. We hypothesised that the pattern and burden of somatic genomic copy number alterations (CNAs) in LGD lesions could predict future AN risk. DESIGN: We performed a retrospective multicentre validated case-control study using n=270 LGD samples from n=122 patients with UC. Patients were designated progressors (n=40) if they had a diagnosis of AN in the ~5 years following LGD diagnosis or non-progressors (n=82) if they remained AN-free during follow-up. DNA was extracted from the baseline LGD lesion, low-coverage whole genome sequencing performed and data processed to detect CNAs. Survival analysis was used to evaluate CNAs as predictors of future AN risk. RESULTS: CNA burden was significantly higher in progressors than non-progressors (p=2×10 CONCLUSION: Measurement of CNAs in LGD is an accurate predictor of AN risk in inflammatory bowel disease and is likely to support clinical management.