Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding.

• Tác giả: Tata Gopinath, Alyssa Kraft, Francesca M Marassi, Kyungsoo Shin, Nicholas A Wood
• Ngôn ngữ: eng
• Ký hiệu phân loại: 070.48346 Journalism
• Thông tin xuất bản: Netherlands : Journal of biomolecular NMR , 2025
• Bộ sưu tập: NCBI
• ID: 177552

Tóm tắt

The NMR signals from protein sidechains are rich in information about intra- and inter-molecular interactions, but their detection can be complicated due to spectral overlap as well as conformational and hydrogen exchange. In this work, we demonstrate a protocol for multi-dimensional solid-state NMR spectral editing of signals from basic sidechains based on Hadamard matrix encoding. The Hadamard method acquires multi-dimensional experiments in such a way that both the backbone and under-sampled sidechain signals can be decoded for unambiguous editing in the