Dentin, a complex, living, and porous mineral substance, is produced by the mineralization of predentin, which is secreted by odontoblasts. This substance is crucial for maintaining the health of teeth. However, the specific function of the vitamin D receptor (Vdr) in the mineralization of odontoblasts, dentin homeostasis, and its interaction with Wnt signaling pathway during dentin apposition is not well understood. In this study, we employed Vdr transgenic knockout mice to study the dental effects and observed enlarged pulp cavities, diminished dentin, and increased predentin thickness in Vdr