Molecular fabrics with fascinating physical characteristics, such as structural flexibility and single-layered thinness, have attracted much attention. Chemists worldwide have been working on building unique molecularly woven structures in two dimensions. However, the synthesis of two-dimensional molecular weaving remains a challenging task, especially in water. Herein, we propose a straightforward and practical method to construct 2D molecular fabrics by enzymatically covalent and noncovalent syntheses in water. In particular, aromatic helical pentamers with two-terminal tyrosine residues (