Metal Ion-Based Chemical Coordination Amplification: A Chromophore In Situ Deposition Strategy for Visual Sensitivity-Enhanced Lateral Flow Immunochromatography Assays.

• Tác giả: Jianjun Dai, Qiannan Hu, Yanmin Ju, Xiangming Meng, Jiaren Song, Changdi Xu, Qing Yang, Jinjun Ye, Siqi Zeng, Wanchao Zuo
• Ngôn ngữ: eng
• Ký hiệu phân loại: 362.14 Professional home care
• Thông tin xuất bản: United States : Analytical chemistry , 2025
• Bộ sưu tập: NCBI
• ID: 178247

Tóm tắt

Traditional lateral flow immunochromatography assays (LFIAs) have faced low sensitivity for trace detection due to the lack of colorimetric brightness. The current strategies to improve sensitivity commonly have the disadvantages of an uncontrollable enhancement process or high background interference, leading to huge obstacles for signal readout. Herein, an in situ metal ion-based chemical coordination amplification (MICCA) strategy has been reported. Metal ion clusters on metal-organic frameworks could coordinate with chromophores to produce colored complexes for visual signal enhancement. A Zr-based metal AIEgen framework (MAF) loaded with Prussian blue was chosen as the dual-mode signal tag for colorimetric and fluorescent readout. MAF could be employed as a grafting substrate to in situ deposit chromophores through the coordination with Zr