Combustion ion chromatography (CIC) has emerged as a valuable tool for determining fluorine content attributable to per- and polyfluoroalkyl substances (PFAS) in biological and environmental samples. The total organofluorine (TOF) data from CIC complement information obtained by targeted analyses using liquid chromatography-tandem mass spectrometry (LC-MS/MS) because TOF includes PFAS that may not be captured by targeted techniques. However, the effect of different PFAS types (chain lengths, head groups, etc.) and matrices on combustion efficiency has not been systematically evaluated. Comparison of C