Biflavonoids, a distinctive subclass of plant flavonoids, have a unique dimerized structure and possess a range of biological activities. The clinical applications of biflavonoids in human health have been impeded by challenges related to bioavailability and hydrophilicity. In contrast, biflavonoid glycosides, which demonstrate enhanced pharmacodynamic and pharmacokinetic properties compared to their aglycones, are notably limited in availability. In this work, we developed a robust enzymatic system to biosynthesize biflavonoid glycosides using