AIM: To study the differential presence of amyloid-β and bacterial lipopolysaccharide (LPS) in freshly extracted titanium implants, either affected by peri-implantitis (PI) or explanted by other causes, and to address a method for removal LPS and amyloid-β from contaminated surfaces. METHODS: Twenty-four explanted implants were harvested from patients with (n = 12) or without (n = 12) peri-implantitis, and their surfaces were analyzed by attenuated total reflectance (ATR) and Fourier transform infrared spectroscopy (FTIR) to localize amyloid-β and LPS. Presence of amyloid-β on the implants surfaces was further analyzed by light microscopy after specific amyloid staining with Congo red. Titanium discs were contaminated with LPS and amyloid-β, these discs as well as six contaminated implants were treated with 0.25% NaOCl to assess its decontamination ability. RESULTS: LPS and amyloid-β were observed at PI affected implant surfaces, but not in implants extracted by other causes. 0.25% NaOCl application was an efficient method for removing LPS and amyloid-β from titanium surfaces. CONCLUSIONS: The concurrent presence of LPS and amyloid-β on the surface of implants affected by PI was demonstrated and it may act as potential comediators of PI inflammatory process. Eliminating these products from implants surfaces is possible after a proteolytic agent (0.25% NaOCl) application.