Carvone is an important flavoring substance and widely used in the food and chemical industries. Carvone has two optical isomers (L-carvone and D-carvone), which have different aroma characteristics. However, the green extraction of natural carvone and isolation of chiral carvone present challenges, leading to its limited application. In this study, a deep eutectic solvent (DES) was prepared and used as a green extraction solvent for the extraction of carvone from herbaceous plants, and then a chiral chromatography column combined with GC-MS was used for the separation and detection of L-carvone and D-carvone. Response surface methodology was used to optimize the extraction conditions including the volume of DES, extraction time and extraction temperature. The results showed that the extraction recoveries and intra-day precision (