The herbicide glyphosate is widely used in agricultural practices around the world, can reach aquatic environments, and potentially impact non-target organisms. This study aimed to investigate the effects of glyphosate exposure (both as the active ingredient and its formulated product) on sperm quality, fertilization success, and development of pejerrey (Odontesthes bonariensis), a native freshwater fish species from Argentina. Results revealed a statistically significant increase in sperm motility at the highest concentration of the formulated product. In contrast, exposure to the active ingredient resulted in a decrease in certain motility parameters. Fertilization assays and embryonic development showed no notable effects in exposed groups. There were no effects in the morphology or temporal evolution of the embryonic stages, nor in the hatching rate. In contrast, larvae exposed to the formulated product exhibited a significant increase in mortality rates, reaching 100% mortality at the highest concentration within a few hours. These findings suggest differential susceptibility between embryos and larvae to glyphosate exposure and highlight the importance of simultaneously assessing the impacts of both the active ingredient and the entire formulation of glyphosate on freshwater fish reproduction and development.